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5a demonstrated that BPC 157 could induce a quick increase of Src phosphorylation at 30 and 60 min with the amount of total Src remained constant

Muhoberac, B

The sections were then washed three times in TNT buffer (0.1 M TrisHCl, 0.15 M NaCl, 0.05% Tween20, pH 7.5) for 5 min, and treated with secondary antibodies